Turn your microscope into a whole-slide scanner. PixelScan WSI stitches the images from your microscope camera into one large, sharp slide image. You can view, measure and annotate it, then export it in the formats that pathology software opens directly. You don't need a motorised stage or an expensive slide scanner: move the slide by hand, and PixelScan WSI places every frame where it belongs. It's made for students, educators, hobbyists and anyone who wants to digitise glass slides with the microscope they already have. It works especially well for brightfield slides such as H&E, special stains and IHC.

Live stitching from a USB microscope camera: move the slide by hand and every frame is placed automatically Build whole-slide images from a video of the slide or from a folder of hundreds or thousands of tiles Sub-pixel placement and global alignment for accurate, seamless mosaics without drift Multi-band blending and flat-field correction for invisible seams and even illumination Fast, Balanced and Best quality stitching modes Any slide size: the mosaic is kept on disk, so gigapixel scans run on an ordinary PC Automatic crash recovery: the scan is saved every 30 seconds Export Aperio SVS, OME-TIFF and pyramidal TIFF that open in QuPath and other pathology software Pixel size, magnification, label and macro images are stored in exported slides Estimated file size before exporting, with JPEG, JPEG 2000, WebP and lossless compression Calibrate each objective with a stage micrometer for true measurements in µm Fast whole-slide viewer for SVS, NDPI, MRXS, SCN, OME-TIFF and pyramidal TIFF Measure length, area, perimeter and angle in µm and mm² QuPath-compatible annotations: rectangles, polygons, freehand, points and text Haematoxylin, eosin and DAB stain separation and Macenko stain normalisation Navigator, magnification buttons from 1.25x to 40x, and a live scale bar Live position marker shows where the camera is on the mosaic and warns when you're lost Progress bar with time left and Cancel for stitching, export and saving Limited Support For UVC/DirectShow cameras, plus drivers for Olympus DP, ToupTek, Basler and more

PixelScan WSI 2.0 • Scans of any size: only part of the mosaic stays in memory; the rest is kept on disk. • Crash recovery: your scan is saved every 30 seconds and can be recovered after a crash. • More accurate stitching: sub-pixel placement, global alignment, multi-band blending, flat-field correction, and Fast/Balanced/Best modes (batch stitching up to 3× faster). • Live stitching runs in the background. The mosaic shows where the camera is and warns you when to move back. • New Export Slide dialog: Aperio SVS, OME-TIFF, pyramidal TIFF, JPEG and PNG, with pixel size, label and macro images, and an estimated file size. • Objective calibration, including stage micrometer measurement. • New slide viewer for SVS, NDPI, MRXS and OME-TIFF, with navigator, measurements, QuPath-compatible annotations and stain separation. • Progress bar with Cancel for stitching, export and saving. • Shortcuts: Space (capture), Ctrl+Z (undo frame), Ctrl+S (save). • Arabic and other non-English file names now work. • Asks to save before closing. Note: projects saved with older versions can't be opened in 2.0.