TBtools-II is a user-friendly bioinformatics platform designed for biologists without programming expertise. Its graphical interface enables intuitive data analysis through menus and parameter settings. Below is a plaintext overview of its core interface-driven features: ​​1. Sequence Processing​​ ​​Batch Operations​​: Extract specific sequences from FASTA files by ID, truncate regions, or reverse-complement sequences with one-click actions. Real-time statistics (sequence length, GC content) are displayed during processing. ​​Gene Structure Analysis​​: Automatically extract gene features (exons, promoters) from GFF/GTF files. Predict open reading frames (ORFs) and translate sequences into protein sequences for export. ​​2. Comparative Genomics​​ ​​BLAST Visualization​​: Configure local BLAST parameters (e.g., E-value thresholds) via sliders and dropdown menus. Results are displayed as dot plots, alignment matrices, or phylogenetic trees. ​​Synteny Analysis​​: Use One-Step MCScanXto detect genome-wide collinearity. Interactive dot plots visualize chromosomal rearrangements and gene duplication events across species. ​​3. Omics Data Analysis​​ ​​Transcriptomics​​: Normalize RNA-seq data (e.g., TMM scaling) and identify differentially expressed genes (DEGs) through volcano plots. Generate GO/KEGG enrichment charts with adjustable color schemes. ​​Heatmaps & Circos Plots​​: Create dynamic heatmaps with clustering options. Circos plots visualize chromatin interactions, gene density, and chromosomal synteny in a few clicks. ​​4. Visualization Tools​​ ​​Dynamic Adjustments​​: Modify heatmap clusters, phylogenetic tree layouts, and Circos tracks in real time. Export high-resolution images (PNG/SVG) for publications. ​​Interactive Editors​​: Edit phylogenetic tree node colors and branch lengths. Adjust Venn/Upset plot overlaps and labels.